Isolation of functional total RNA from Argemone mexicana tissues

Cytotoxic benzophenanthridine and benzylisoquinoline alkaloids from Argemone mexicana.

Fractionation of the chloroform extract from the aerial part of Argemone mexicana led to the isolation of two benzophenanthridine-type alkaloids, N-demethyloxysanguinarine and pancorine; three benzylisoquinoline-type alkaloids, (+)-1,2,3,4-tetrahydro-1-(2-hydroxymethyl-3,4-dimethoxyphenylmethyl)-6,7-methylenedioxyisoquinoline, (+)-higenamine and (+)-reticuline. Among them, N-demethyloxysanguina...

RNA isolation (Total RNA Extraction from High Polysaccharide Plants). Fatemeh Mehrpooyan*

Neuropharmacological study of Argemone mexicana Linn

In the present study methanolic and ethyl acetate extracts of Argemone mexicana whole plant (Papaveraceae) were tested orally in swiss albino mice at doses of 100 mg/kg, 200 mg/kg and 400 mg/kg b.w. for CNS related activities. Papaveraceae family is known to have CNS depressant activity, so A. mexicana was evaluated for CNS activities. Significant central and peripheral nociceptive activity was...

n-alkane profile of Argemone mexicana leaves.

An n-hexane extract of fresh, mature leaves of Argemone mexicana (Papaveraceae), containing thin-layer epicuticular waxes, has been analysed for the first time by TLC, IR and GLC using standard hydrocarbons. Seventeen long-chain alkanes (n-C18 to n-C34) were identified and quantified. Nonacosane (n-C29) was established as the n-alkane with the highest amount, whilst octadecane (n-C19) was the l...

High Quality RNA Isolation from Leaf, Shell, Root Tissues and Callus of Hazelnut (Corylus avellana L.)

Extraction of high quality RNA is a critical step in molecular genetics studies. Hazelnut is one of the most important nuts plants in the world. The presence of the taxol and other taxanes in hazelnut plant necessitates explaining their biosynthesis pathway and identifying the candidate genes. Therefore, an easy and practical method is necessary for RNA extraction from hazelnuts. Hazelnut has h...